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EFFECTS OF LIDOCAINE ON RABBIT BRAIN MICROTUBULAR PROTEIN 1
Author(s) -
Haschke R. H.,
Byers Margaret R.,
Fink B. Raymond
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04302.x
Subject(s) - lidocaine , microtubule , procaine , electron microscope , polymerization , chemistry , biophysics , tubulin , chromatography , biology , pharmacology , microbiology and biotechnology , neuroscience , organic chemistry , physics , optics , polymer
Microtubule repolymerization from a crude supernatant fraction prepared from rabbit brain was followed quantitatively by viscometry and electron microscopy. Lidocaine inhibits this repolymerization in a dose‐dependent fashion and the effect is completely reversible upon removal of the lidocaine by dialysis. Direct counting of microtubules by electron microscopy indicates that the local anesthetic decreases the number of tubules without significantly affecting their length. An association constant of lidocaine for tubulin is estimated at 7.5 m m . Procaine and etidocaine were also found to affect the polymerization of microtubules with results similar to those found with lidocaine.