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DDT EFFECT ON 32 P INCORPORATION FROM γ‐LABELLED ATP INTO PROTEINS FROM LOBSTER NERVE
Author(s) -
Doherty J. D.,
Matsumura F.
Publication year - 1974
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1974.tb04292.x
Subject(s) - ouabain , chemistry , microsome , ethylene , biochemistry , sodium , in vitro , organic chemistry , catalysis
The insecticide DDT (1,1,1 trichloro‐2,2‐bis‐(p‐chlorophenyl) ethane) was found to affect the amount of 32 P from [γ‐ 32 P] ATP incorporated into proteins derived from lobster peripheral nerves in a variety of ways depending upon the relative concentrations of ATP, Na + , K + , Mg 2+ , and Ca 2+ . When a high concentration of ATP (2.5 ± 10 −5 m ) was used DDT (10 −5 m ) inhibited the incorporation and reversed the increased incorporation caused by ouabain. At low concentration of ATP (8.6 ± 10 −8 m ), DDT inhibited the incorporation when the buffer contained Mg 2+ and Na + or K + alone, but when the buffer contained Mg 2+ and both Na + and K + together, DDT consistently caused an increased amount of 32 P to be incorporated. The ability of DDT to cause increases in incorporation of 32 P into the lobster nerve proteins was found to parallel many of the properties that this compound shows when allowed to poison crustacean nerves. For example: the effect was negatively temperature dependent, Ca 2+ lowered the incorporation in the presence of DDT, and DDT consistently caused greater amounts of 32 P to be incorporated than its less potent analog DDE (1,1‐dichloro‐2,2‐ bis ‐(p‐chlorophenyl)ethylene). The proteins that were affected by DDT were microsomal in nature and could be centrifuged from supernatant by recentrifuging at 149,000 g. The possibility that this system may be the actual target through which DDT causes its characteristic interferences of the ionic conductance changes associated with the action potential are discussed.