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EFFECTS OF AMINO‐OXYACETIC ACID ON [ 3 H]GABA UPTAKE BY RAT BRAIN SLICES
Author(s) -
Snodgrass S. R.,
Iversen L. L.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb12142.x
Subject(s) - amino acid , pyridoxine , glutamate receptor , transaminase , inhibitory postsynaptic potential , gaba transaminase , chemistry , aminooxyacetic acid , pyridoxal phosphate , gaba transporter , glycine , biochemistry , aminobutyric acid , gamma aminobutyric acid , medicine , endocrinology , glutamate decarboxylase , biology , neurotransmitter , enzyme , receptor , cofactor
— The effect of amino‐oxyacetic acid on the uptake of [ 3 H]GABA by rat brain slices was studied. When added simultaneously with [ 3 H]GABA, amino‐oxyacetic acid had no significant effect on [ 3 H]GABA uptake. However, preincubation of brain slices with amino‐oxyacetic acid prior to addition of [ 3 H]GABA produced inhibition of uptake, which increased with longer duration of preincubation. The inhibitory effect of amino‐oxyacetic acid was maximal at 2 mM concentration and concentrations sufficient to inhibit significantly GABA:glutamate transaminase (10 ‐ ‐ 6 M) had no effect on [ 3 H]GABA uptake. D‐Cycloserine and β‐hydrazino‐propionic acid also inhibited [ 3 H]GABA uptake, but the amounts required were considerably in excess of those needed to inhibit GABA:glutamate transaminase. 4‐Deoxypyridoxine inhibited [ 3 H]GABA uptake, whether given in vivo or in vitro , and the inhibitory effect of amino‐oxyacetic acid was reversed with pyridoxine. GABA transport appears to be dependent on pyridoxal phosphate and interference with this function of the vitamin is suggested as the basis for the inhibitory effect of amino‐oxyacetic acid on [ 3 H]GABA uptake.