Premium
KINETICS OF CHOLINE ACETYLTRANSFERASES (EC 2.3.1.6) FROM HUMAN AND OTHER MAMMALIAN CENTRAL AND PERIPHERAL NERVOUS TISSUES
Author(s) -
White Helen L.,
Wu Joyce Chen
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb12129.x
Subject(s) - acetyltransferases , choline , sciatic nerve , choline acetyltransferase , central nervous system , chemistry , in vivo , peripheral nervous system , caudate nucleus , nicotinamide , biochemistry , endocrinology , biology , medicine , anatomy , enzyme , microbiology and biotechnology , gene , acetylation
— Choline acetyltransferase (EC 2.3.1.6) was partially purified from human caudate nucleus and putamen, human sciatic nerve, rabbit and rat brain, and rabbit sciatic nerve. Kinetic constants were determined under the same conditions for all six extracts. Extrapolated K m values were between 6.6 and 18 μM for acetyl‐CoA and between 0.4 and 1.2 mM for choline. Product inhibition patterns indicated that ChAc from both central and peripheral nervous tissues of man and the rabbit obeys a Theorell‐Chance mechanism. Kinetic parameters suggest a possible influence on ACh synthesis of the in vivo concentration ratio, CoA/acetyl‐CoA.