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EFFECTS OF ANOXIA IN VITRO ON CELLULAR RESPIRATION OF BRAIN CORTEX
Author(s) -
Phizackerley P. J. R.,
Fixter L. M.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb12110.x
Subject(s) - anaerobic exercise , glutamine , respiration , cortex (anatomy) , cerebral cortex , in vitro , hypercapnia , carbohydrate metabolism , cellular respiration , biology , saline , metabolism , glucose uptake , chemistry , biochemistry , medicine , endocrinology , acidosis , anatomy , neuroscience , physiology , amino acid , insulin
— –Anaerobic preincubation of slices of brain cortex from adult rats in saline at 37°C, but not at 20°C, impaired their capacity to oxidize glucose and other substrates. This effect was to a large extent prevented by including either glucose + oxaloacetate, or l ‐glutamine + l ‐aspartate +α‐oxoglutarate in the medium during anaerobic preincubation, and in addition the anaerobic uptake of water by the tissue was decreased. The respiratory mechanism in slices of brain cortex from 1‐day‐old rats was much less affected by anoxia and sensitivity to anoxia develops with age.