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DEVELOPMENT OF Na + ‐STIMULATED GLUCOSE OXIDATION IN SYNAPTOSOMES 1, 2
Author(s) -
Diamond I.,
Fishman R. A.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb07558.x
Subject(s) - ouabain , chemistry , carbohydrate metabolism , choline , metabolism , sodium , biochemistry , medicine , organic chemistry
— d ‐[ 14 C]Glucose was oxidized to 14 CO 2 by synaptosomes prepared from adult rat brain. Added Na + stimulated glucose oxidation by 179%, but K + and choline were without effect. Li + stimulated glucose oxidation by 64%. Ouabain largely prevented the stimulatory effect of Na + on glucose oxidation but had no effect in the absence of Na + . 2‐Deoxy‐ d ‐glucose competitively inhibited glucose oxidation differently at two different ranges of deoxyglucose and glucose concentrations; the K i was 0.54 and 16 m m , respectively. In the presence or absence of Na + 2,4‐DNP‐stimulated glucose oxidation by 370% while iodoacetate inhibited glucose oxidation by 87–95%. There was a striking increase in Na + ‐stimulated glucose oxidation with development but glucose oxidation in the absence of Na + did not change dramatically with age. Taken together the data suggest synaptosomes exhibit coupled respiration which can be modulated by Na + . In addition, the appearance of Na + ‐stimulated glucose oxidation with maturation probably is linked to the development of Na + ‐K + ‐ATPase acitivity in the synaptosomal membrane.