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GLYCERIDE METABOLISM IN CULTURED CELLS DISSOCIATED FROM RAT CEREBRAL CORTEX 1
Author(s) -
Yavin E.,
Menkes J. H.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb07535.x
Subject(s) - glycerol , biochemistry , stearate , metabolism , glyceride , fatty acid , choline , stearic acid , tritium , phospholipid , lipid metabolism , cell culture , chemistry , membrane , biology , organic chemistry , genetics , physics , nuclear physics
— [1‐ 14 C]stearic acid and [2‐ 3 H]glycerol were rapidly taken up and esterified into triacylglycerol and phospholipids by rat brain cells cultivated in monolayers. Expressed in terms of pool size, the incorporation of glycerol and stearate into triacylglycerol was 6‐ and 8‐fold, respectively, higher than the incorporation into the choline phosphoglycerides. Tritium‐labelled glycerol in both triacylglycerol and glycerophosphatides was diluted more rapidly than the [ 14 C] labelled fatty acids. Chase experiments indicated a transfer of fatty acid from one lipid class to another, mainly from triacylglycerol to phospholipids, with no apparent loss of radioactivity. The accumulation of triacylglycerol in the brain cells was a function of both the presence of exogenous fatty acids in the culture medium and the metabolic needs of the cells; as long as the cells were involved in active formation of membranes the proportion of triacylglycerol was relatively small; its concentration increased while cell division slowed down in older, fully monolayered cultures.