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SOME FACTORS AFFECTING THE UPTAKE, BINDING AND RETENTION OF [ 3 H]CORTISOL BY THE CHICK EMBRYO RETINA AS RELATED TO ENZYME INDUCTION
Author(s) -
Chader G. J.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb06036.x
Subject(s) - cytosol , steroid , incubation , medicine , endocrinology , retina , biology , embryo , glucocorticoid , corticosterone , metabolism , receptor , biochemistry , glutamine synthetase , glutamine , enzyme , hormone , microbiology and biotechnology , amino acid , neuroscience
—The accumulation of [ 3 H]cortisol by the embryonic chick neural retina was studied at a time of rapid biochemical differentiation. In retinal cultures, uptake of steroid was decreased by inhibitors of energy metabolism and of sulphhydryl groups but not by inhibitors of macromolecular synthesis. The retention of steroid was inhibited by dinitrophenol and N ‐ethyl maleimide (NEM) over a 3 h incubation period. This inhibition may be due, in part, to the effect of NEM on the binding of steroid to intraretinal receptor as can be demonstrated by gel filtration. Neuraminidase had no effect on [ 3 H]cortisol uptake or retention but markedly inhibited the induction of glutamine synthetase by the steroid. Uptake and retention of steroid at 37° was greater in the retinal nuclear pellet than in the cytosol fraction; the reverse was true at 4°. After treatment with NEM, [ 3 H]cortisol accumulation in the nuclear pellet was drastically decreased, with approximately the same level of uptake as that seen at 4°. A temperature‐dependent, sulphhydryl‐sensitive process of steroid translocation from cytoplasm to nucleus may thus be indicated.

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