Premium
ZUR ENERGIEBEDÜRFTIGEN Ca 2+ ‐AKKUMULATION IN MITOCHONDRIEN AUS RATTENHIRN
Author(s) -
Böhme G.,
Lutze G.,
Pollak K.H.,
Winter O.,
Thews J.,
Kassebaum I.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb06034.x
Subject(s) - mitochondrion , antimycin a , adenine nucleotide , chemistry , respiration , incubation , nucleotide , biochemistry , biology , anatomy , gene
—(1) The properties of a preparation of functional intact rat brain mitochondria are described and the resulting fraction is enzymatically characterized. (2) These mitochondria are able to accumulate Ca 2+ in a respiration‐dependent reaction without additions of P i and/or adenine nucleotides. (3) As criteria the increased acidity of the incubation medium accompanying the energy‐dependent Ca 2+ accumulation, its inhibition by rotenon and Antimycin A, the acceleration of respiration and the redox change of the pyridine nucleotides were recorded and Ca 2+ accumulation by the mitochondria was determined by complexemetric methods. (4) The maximum Ca 2+ accumulation by rat brain mitochondria amounts to only 25 per cent of that by rat liver mitochondria under similar conditions (on the base per mg protein); after addition of 1 m m ‐ADP (without P i ) the comparable value was about 50 per cent.