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CHARACTERISTICS OF THE CATECHOLAMINE AND HISTAMINE RECEPTOR SITES MEDIATING ACCUMULATION OF CYCLIC ADENOSINE 3′,5′‐MONOPHOSPHATE IN GUINEA PIG BRAIN 1
Author(s) -
Chasin M.,
Mamrak F.,
Samaniego Sylvia G.,
Hess S. M.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb06026.x
Subject(s) - medicine , endocrinology , epinephrine , histamine , adenosine , catecholamine , chemistry , stimulation , hippocampus , cerebral cortex , adrenergic , adrenergic receptor , receptor , biology
—Five areas of guinea pig brain were examined to determine the properties of the receptor sites mediating increases in [ 3 H]adenosine 3′,5′‐monophosphate (cyclic AMP). Both epinephrine and histamine were effective in causing increases in cyclic AMP in slices derived from cerebral cortex, hippocampus or amygdala, but not in diencephalon or brainstem. Stimulation of slices of cerebral cortex by either epinephrine or histamine resulted in a small, but reproducible, decrease in specific radioactivity of the [ 3 H]‐cyclic AMP produced, as did stimulation of the hippocampus by epinephrine. The catecholamine receptor was an α‐adrenergic receptor in all three areas where epinephrine was effective; α‐adrenergic stimulation, but not β‐adrenergic stimulation, increased levels of [ 3 H]‐cyclic AMP. Furthermore, α‐, but not β‐adrenergic blocking agents, prevented the epinephrine‐ induced increase of both [ 3 H]‐ and total cyclic AMP in cerebral cortex and hippocampus. Only antihistaminic agents were capable of antagonizing the histamine‐induced increase of both [ 3 H]‐ and total cyclic AMP in these two brain areas. The catecholamine receptor in the amygdala also appeared to be an α‐adrenergic receptor. The effects of histamine and epinephrine together were far greater than the sum of effects of either hormone alone in both cerebral cortex and hippocampus.