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AN ASSAY PROCEDURE FOR TRYPTAMINE IN BRAIN AND SPINAL CORD USING ITS [ 3 H]DANSYL DERIVATIVE
Author(s) -
Snodgrass S. R.,
Horn A. S.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb06013.x
Subject(s) - tryptamine , chemistry , chromatography , dansyl chloride , tryptophan , spinal cord , pargyline , monoamine oxidase , biochemistry , derivatization , amino acid , high performance liquid chromatography , biology , enzyme , neuroscience
—The tryptamine content of rat and mouse brain and spinal cord was determined with a radiochemical derivative assay, using [ 3 H]dansyl chloride. The amine was extracted into toluene‐isoamyl alcohol, back‐extracted into dilute acid, then adsorbed onto a non‐ionic polystyrene resin, and dansylated in tetrahydrofuran after elution from the resin. Optimum recoveries were obtained with TCA extracts, although significant losses occurred due to surface adsorption and protein binding. The brain content of tryptamine increased after MAO inhibition and was not significantly further increased when tryptophan loading was combined with inhibition of MAO and/or tryptophan 5‐hydroxylase. The tryptamine concentration of spinal cord exceeded that of brain and increased rapidly after death. Among brain regions tryptamine concentrations were greatest in hypothalamus and striatum and lowest in cerebral cortex and cerebellum.