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ISOLATION OF PURIFIED BASIC PROTEIN FROM HUMAN BRAIN
Author(s) -
Banik N. L.,
Davison A. N.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb05994.x
Subject(s) - myelin basic protein , polyacrylamide gel electrophoresis , myelin , chromatography , electrophoresis , biochemistry , chemistry , isolation (microbiology) , chloroform , biology , enzyme , bioinformatics , neuroscience , central nervous system
—A simplified method for the isolation of an encephalitogenic basic protein from myelin is described. The basic polypeptide is easily extracted from the interfacial protein which separates when chloroform‐methanol extracts of myelin are treated with a synthetic upper phase containing citrate. Evidence is presented for the purity and identity of the protein. The disc polyacrylamide gel electrophoretic pattern of this protein was consistent with a high degree of homogeneity.