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Inhibition of the uptake of 5‐hydroxytryptamine, noradrenaline and dopamine into rat brain homogenates by various hydroxylated tryptamines
Author(s) -
Hoax A. S.,
Baumgarten H. G.,
Schlossberger H. G.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb04242.x
Subject(s) - tryptamines , tryptamine , dopamine , chemistry , striatum , synaptosome , serotonin , free nerve ending , medicine , endocrinology , pharmacology , biochemistry , biology , in vitro , receptor
Recent work has shown that intracerebral injections of 5,6‐dihydroxytryptamine (5,6‐DHT) lead to a fairly selective and long lasting depletion of 5‐HT in the rat CNS (BAUMGARTEN, BJORKLUND, LACHENMAYER, NOBIN and STENEVI, 1971; DALY, FUXE and JONSSON, 1973). This effect appears to result from a degeneration of the serotonin‐containing neurons (BAUMGARTEN and LACHENMAYER, 1972a). 5,6‐DHT does, however, to a lesser extent affect both NA and dopamine (DA) containing nerve terminals (BAUMGARTEN et al., 1971). In an attempt, therefore, to find compounds having a more specific toxic action we have investigated several other hydroxylated tryptamines. In order to obtain information about the differential affinities of these analogues for neuronal uptake sites we have examined their effects on the uptake of [ 3 H]5‐HT and (±)‐[ 3 H]NA into synaptosomes in homogenates of rat hypothalamus and of [ 3 H]DA uptake into a similar preparation from the rat corpus striatum. It is known that the uptake of these putative transmitters in rat brain homogenates is predominantly into the synaptosome fraction (KANNENGIESSER, HUNT and RAYNAUD, 1973; COYLE and SNYDER, 1969).