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AXOPLASMIC TRANSPORT OF AROMATIC l ‐AMINO ACID DECARBOXYLASE (EC 4.1.1.26) AND DOPAMTNE β‐HYDROXYLASE (EC 1.14.2.I) IN RAT SCIATIC NERVE
Author(s) -
Dairman W.,
Geffen L.,
Marchelle Mary
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00278.x
Subject(s) - aromatic l amino acid decarboxylase , chemistry , axoplasmic transport , dopamine , enzyme , biochemistry , amino acid , aromatic amino acids , norepinephrine , tyrosine hydroxylase , biosynthesis , endocrinology , biology , anatomy
The axoplasmic transport of aromatic l ‐amino acid decarboxylase and dopamine β‐hydroxylase, two enzymes involved in the biosynthesis of catecholamines, was studied in rat sciatic nerve. The two enzymes exhibited markedly different axoplasmic flow characteristics, since dopamine β‐hydroxylase activity accumulated on the proximal side of a ligation nearly three times as fast as aromatic l ‐amino acid decarboxylase activity. Distally dopamine β‐hydroxylase activity remained essentially constant for 24 h, whereas aromatic l ‐amino acid decarboxylase activity fell precipitously. Evidence was obtained to rule out the possibility that differences in the rate of inactivation of the two enzymes could account for the different rates of accumulations observed. The conclusion, that aromatic L‐amino acid decarboxylase and dopamine β‐hydroxylase are transported in sympathetic nerve at different rates is discussed in relation to the biosynthesis of norepinephrine.