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Effect of growth hormone on glycogenesis in cerebral cortical slices
Author(s) -
Visweswaran P.,
Subrahmanyam K.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00265.x
Subject(s) - glycogenesis , glycogen , endocrinology , medicine , insulin , stimulation , glycogen synthase , alloxan , bovine somatotropin , carbohydrate metabolism , chemistry , hormone , metabolism , biology , growth hormone , diabetes mellitus
T he uptake of glucose by cerebral cortical slices of rats was found to be enhanced by insulin by R afaelsen (1961) and G enes and C harnaya (1966). This was confirmed by P rasannan and S ubrah‐manyam (1965) and more recently by N elson , S chultz , P asoneau and W ry (1968). E isenberg and S eltzer (1962) and G otistein , H eld , S ebenng and W alpurger (1965) obtained evidence for a direct effect of insulin on the entry of glucose into brain and on its metabolism in this tissue. A marked resynthesis of glycogen was demonstrated with glucose as substrate by L ebaron (1955) and M cilwain and T resize (1956) in cerebral cortical slices of the guinea pig. P rasannan and S ubrahmanyam (1965) obtained evidence for a similar resynthesis of glycogen in cerebral cortical slices of the rat. Addition of 0.2 unit of insulin per 3.5 ml of incubating medium gave rise to an increase of 60 per cent in the resynthesis of glycogen in these slices. The incorporation of 14 C from labelled glucose into glycogen and CO 2 by cerebral cortical slices of normal and alloxan diabetic rats and the stimulation of the incorporation into glycogen by insulin in vitro was reported by V isweswaran , P rasannan and S ubrahmanyam (1969). An insulin‐like action of growth hormone on the carbohydrate metabolism was reported by K etterer , R andle and Y oung (1967) and M anchester and Y oung (1961). It was believed to be due to the formation of a polypeptide breakdown product of growth hormone which has biological insulin‐like properties. P ark , B rown , C ornbluth , D aughaday and K rahl . (1952) reported an increased uptake of glucose by isolated rat diaphragm due to the action of growth hormone which is similar to that of insulin. Hence, it was considered appropriate to study the incorporation of 14 C from labelled glucose into glycogen and CO 2 by cerebral slices of growth hormone treated rats and the effect of growth hormone treatment on the activities of the enzymes concerned with glycogenesis in rat cerebral cortex.