Premium
An ATP pool associated with adenyl cyclase of brain tissue
Author(s) -
Shimizu H.,
Okayama H.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00098.x
Subject(s) - veratridine , adenosine , histamine , nucleotide , adenosine monophosphate , adenine nucleotide , cyclase , adenylate kinase , chemistry , biochemistry , adenosine triphosphate , cerebral cortex , biophysics , biology , endocrinology , enzyme , sodium , organic chemistry , sodium channel , gene
R adioactive adenine has been frequently used to label cyclic adenosine 3′, 5′‐monophosphate (cAMP) in diverse cells and tissues (K uo and D e R enzo , 1969; S himizu , D aly and C reveling , 1969, B rooker , 1971). We have previously reported that cAMP from the guinea‐pig cerebral cortex is derived from a precursor pool of adenine nucleotides which is more highly labelled than the bulk cellular ATP (S himizu , C reveling and D aly , 1970a). The present study was carried out to throw additional light on the property of the precursor pool. Levels of cAMP and ATP and specific activities of [ 14 C]cAMP and [ 14 C]ATP were determined simultaneously in cerebral slices which were prelabelled with [ 14 C]adenine and subsequently incubated with veratridine, histamine or adenosine.