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STUDIES BY DNA‐RNA HYBRIDIZATION OF TRANSCRIPTIONAL DIVERSITY IN HUMAN BRAIN 1
Author(s) -
Grouse L.,
Omenn G. A.,
McCarthy B. J.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00077.x
Subject(s) - rna , human brain , biology , dna , cerebral cortex , astrocytoma , cortex (anatomy) , atrophy , microbiology and biotechnology , neuroscience , gene , genetics , glioma
— Purified unique sequences of human DNA (from HeLa cells) were hybridized with various preparations of human brain KNA, to obtain estimates of the fraction of DNA transcribed. Values of 12 per cent were obtained for fetal brain increasing to 24 per cent for specific regions of adult brain. Right and left parietal and temporal cortical RNA gave similar plateau values but left frontal cortical RNA appeared to represent 2–3 times as much of the total genome as that from right frontal cortex. Lower values were obtained for fetal and adult brain stem and for RNA prepared from temporal cortex of a patient exhibiting gross cerebral atrophy. Attempts were made to assess the contributions of glial and neuronal cells by using human astrocytoma and mouse neuroblastoma RNA.