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THE EFFECTS OF TETRAPHENYLBORON ON THE ISOLATION OF NEURONAL AND GLIAL CELLS FROM GUINEA‐PIG BRAIN
Author(s) -
Baritch J. A.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00045.x
Subject(s) - guinea pig , carbonic anhydrase , biochemistry , chemistry , biophysics , microbiology and biotechnology , biology , enzyme , endocrinology
— The addition of 20 mM‐tetraphenylboron to the tissue‐softening medium of a neuronal and glial cell isolation procedure results in a 2‐fold and 5‐fold increase in the yield of neuronal and glial‐enriched material respectively. The purity of the isolated fractions appears unaltered. While tetraphenylboron strongly inhibits protein synthesis, carbonic anhydrase activity and structural integrity appear unaffected by tetraphenylboron. The potential use of this compound in cell isolations is discussed.