Premium
ENZYMIC ACTIVITIES IN DISSOCIATED NEURONS DIFFERENTIATED IN CULTURES IN VITRO
Author(s) -
Contestabile A.,
Minelli G.,
Ciani F.
Publication year - 1973
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1973.tb00029.x
Subject(s) - neuroblast , acetylcholinesterase , in vitro , lactate dehydrogenase , biology , succinate dehydrogenase , monoamine oxidase , spinal cord , acid phosphatase , cerebellum , cellular differentiation , enzyme , biochemistry , microbiology and biotechnology , neuroscience , neurogenesis , gene
— The appearance of the enzymes succinate dehydrogenase, lactate dehydrogenase, acid phosphatase, monoamine oxidase and acetylcholinesterase in cultures of dissociated spinal cord, dorsal root ganglia and cerebellum removed at different stages of development have been studied. The neurons differentiated in vitro have morphological and cytochemical characteristics similar to those of the normally developing neurons. On the basis of the results obtained, it is argued that in this experimental model the histogenetic determination of the neuroblasts at the time of the explant may start and bring to an end the morphological and biochemical differentiation of the various types of neurons even under conditions extremely different from those of normal development.