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EFFECTS OF 5‐PHENYLOXAZOLIDINEDIONE ON SODIUM–POTASSIUM–MAGNESIUM‐ACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN MOUSE BRAIN 1
Author(s) -
Brink J. J.,
Freeman Ellen A.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb06223.x
Subject(s) - ouabain , potassium , sodium , adenosine triphosphatase , chemistry , magnesium , atpase , medicine , endocrinology , triphosphatase , biochemistry , enzyme , biology , organic chemistry
—10 μ m 5‐Phenyloxazolidinedione (PKO) stimulated Na–K–Mg‐ATPase activity in mouse brain homogenates by 45%. This activation effect was also observed for trimethadione and dimethadione. Inhibition of Na–K–Mg‐ATPase by 0.1 m m ouabain was reduced in the presence of PKO. When PKO (4 mg/kg) was administered to mice intravenously, maximum levels of 2 μg (10 nmol)/g were attained in the brain within 15 min. PKO had an ld 50 of 872 mg/kg and an ed 50 of 375 mg/kg for protection against metrazol‐induced seizures in mice.