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SUBCELLULAR DISTRIBUTION OF POTASSIUM, SODIUM, MAGNESIUM, CALCIUM AND CHLORIDE IN CEREBRAL CORTEX 1
Author(s) -
Hanig Ruth C.,
Tachiki K. H.,
Aprison M. H.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb05093.x
Subject(s) - potassium , sodium , chemistry , magnesium , calcium , biochemistry , chromatography , inorganic chemistry , organic chemistry
The subcellular distributions of potassium, sodium, magnesium, calcium and chloride have been determined for rabbit cerebral cortex. After homogenization and differential centrifugation, the following percentages of ions were associated with the particulate fraction (nuclear, mitochondrial, synaptic vesicles, and microsomal): (a) 19% of the total potassium; (b) 22% of the total sodium; (c) 77% of the total calcium; (d) 69% of the total magnesium; and (e) less than 2% of the total chloride. However, the sum of the potassium and sodium content in each of the particulate fractions was greater than the sum of the calcium and magnesium content. After hypo‐osmotic shock of the crude mitochondrial fraction ( M T ), more sodium than potassium ( μ mol/g wet wt.) was associated with the mitochondrial ( M 1 ) and synaptic vesicle ( M 2 ) fractions. The molar ratio of sodium to potassium was 1·4 for M 1 and 4·5 for M 2 . The association of 22 Na + with the particulate fractions was further studied by the method of equilibrium dialysis. The data from both types of experiments indicate that a large fraction of the sodium in cortical tissue appears to be in a bound state.