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THE PRESENCE OF BIOLOGICALLY LABILE COMPOUNDS DURING ISCHEMIA AND THEIR RELATIONSHIP TO THE EEG IN RAT CEREBRAL CORTEX AND HYPOTHALAMUS
Author(s) -
Swaab D. F.,
Boer K.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb03822.x
Subject(s) - hypothalamus , cerebral cortex , medicine , endocrinology , glycogen , creatine , cortex (anatomy) , ischemia , chemistry , electroencephalography , in vivo , neuroscience , biology , microbiology and biotechnology
—Two surgical methods are described in the present paper, allowing for the approximate determination of in vivo levels of ATP, lactate, glucose, pyruvate and glycogen in anatomically uninjured cortex and hypothalamus from unanaesthetized rats. It was not possible to obtain such levels for P‐creatine in the 2 mm thick samples used in the present investigation. No fundamental difference was observed between the cortical and the hypo‐thalamic levels of these substrates nor in their fluxes. The substrate fluxes during ischemia were correlated with electrical activity in the rat cortex and hypothalamus, recorded by means of telemetrically transmitted electroencephalograms. The electrical activity declined precipitously at 9.6 s after decapitation in the cortex, and after 12.1 s in the hypothalamus. High levels of glycogen, glucose and ATP were present at this moment, while P‐creatine had declined sharply.