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EFFECTS OF BACTRACHOTOXIN ON ELECTROPLAX Na + ‐K + ‐ATPase AND LEVELS OF ATP IN RAT MUSCLE 1
Author(s) -
Daly J.,
Albuquerque E. X.,
Kauffman F. C.,
Oesch F.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb03820.x
Subject(s) - batrachotoxin , electrophorus , chemistry , phosphocreatine , atpase , depolarization , sarcolemma , fructose , biophysics , biochemistry , membrane , enzyme , medicine , sodium , endocrinology , biology , sodium channel , acetylcholine receptor , receptor , energy metabolism , torpedo , organic chemistry
—Batrachotoxin (BTX) in low concentrations (20 n m ) depolarizes electrically excitable membranes (A lbuquerque , D aly and W itkop , 1971). At these levels, BTX does not inhibit Na + ‐K + ‐ATPase. At much higher concentrations (60 μ m ) BTX partially inhibits Na + ‐K + ‐ATPase from electroplax of Electrophorus electricus. In contrast to inhibition by cardiac glycosides, the inhibition of Na + ‐K + ‐ATPase by batrachotoxin is not antagonized by KCl. BTX had no effect on ATP levels in stimulated nerve muscle preparations at the time when sustained contracture was initiated by the drug. Phosphocreatine levels were decreased and levels of glucose‐6‐phosphate and 6‐phosphogluconate were increased, while levels of fructose‐1,6‐diphosphate and α‐ketoglutarate were unchanged. It is concluded that the inhibition of Na + ‐K + ‐ATPase or lowering of ATP levels by BTX is not significantly involved in the membrane depolarization produced by the toxin.