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RELATION OF ATP AND CREATINE PHOSPHATE TO FAST AXOPLASMIC TRANSPORT IN MAMMALIAN NERVE 1
Author(s) -
Sabri M. I.,
Ochs S.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb03819.x
Subject(s) - axoplasmic transport , iodoacetic acid , creatine , phosphate , chemistry , sciatic nerve , biophysics , biochemistry , biology , anatomy , enzyme
—ATP and creatine phosphate (CP) levels in cat sciatic nerve maintained in vitro were measured. Anoxia produced by N 2 or NaCN or the uncoupling of phosphorylation with DNP reduced the combined levels of ATP + CP to approximately one‐half of control levels within 15 min. These agents also blocked fast axoplasmic transport in vitro within 15 min. A block of glycolysis with iodoacetic acid (IAA) reduced the combined levels of ATP + CP to approximately one half of control levels within 1.5–2 h and exposure of nerve in vitro to IAA caused a block of fast axoplasmic transport within the same interval. The correlation of the time at which block of transport occurred with the fall in the level of high‐energy phosphates is consistent with the hypothesis that ATP supplies the energy required by the mechanism underlying fast exoplasmic transport.