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THE ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF THREE SUBFRACTIONS OF MYELIN FROM CENTRAL NERVOUS TISSUE OF THE ADULT RAT 1
Author(s) -
McMillan P. N.,
Williams N. I.,
Kaufman B.,
Day E. D.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01472.x
Subject(s) - myelin , cerebroside , chromatography , sphingomyelin , chemistry , ultracentrifuge , biochemistry , centrifugation , thin layer chromatography , myelin basic protein , cholesterol , central nervous system , biology , neuroscience
— By techniques of isosmotic density gradient ultracentrifugation three subfractions of myelin were isolated from homogenates of whole rat brain at densities of 1.054 g/ml (myelin I), 1.060 g/ml (myelin II) and 1.066 g/ml (myelin III). The stability of these fractions was demonstrated by the zonal centrifuge profile analysis of recycled fractions. Examination of the three myelin subfractions by techniques of electron microscopy and thin layer chromatography detected no obvious morphological or chemical differences. However, analysis for protein, cholesterol, phospholipids and cerebrosides did reveal differences among myelin I, myelin II and myelin III. Myelin I contained relatively more cholesterol than II or III. Myelin III contained relatively more phospholipids than I or II. The cerebroside‐to‐protein ratios were the same in all three fractions. Quantitative differences in fatty acid composition (as detected by gas‐liquid chromatography) were also observed.