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INHIBITION OF CHOLINE ACETYLTRANSFERASE AND ITS HISTOCHEMICAL LOCALIZATION
Author(s) -
Kása P.,
Morris D.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01455.x
Subject(s) - choline acetyltransferase , acetyltransferase , enzyme , biochemistry , choline , staining , enzyme assay , hydrolysis , chemistry , biology , microbiology and biotechnology , acetylation , acetylcholine , endocrinology , genetics , gene
— A method for the histochemical identification of choline acetyltransferase has been investigated further by studying the effects of certain inhibitors of the enzyme both on rat brain homogenates and on the localization of the enzyme in tissue sections. It was confirmed that acetyl‐CoA hydrolase activity both in homogenates and in tissue sections is inhibited by preincubation in 1 mM‐DFP. The effects of the choline acetyltransferase inhibitors chloro‐ and bromoacetylcholine on the appearance of histochemical staining were related to their activity in homogenates and tissue slices. Bromoketone was found to inhibit choline acetyltransferase in homogenates and, less efficiently, in tissue sections but it also inhibited the hydrolysis of acetyl‐CoA by some other unknown enzyme which is inactivated by 1 mM‐DFP. The results obtained with the choline acetyltransferase inhibitors provide support for the specificity of the histochemical method.