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DIFFERENTIATION OF CHICK EMBRYO CEREBRAL HEMISPHERES: A CRITICAL EYALUATION OF A BULK PREPARATION OF NEUROBLASTS AND SPONGIOBLASTS
Author(s) -
Judes C.,
Jacob M.,
Kister L.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01435.x
Subject(s) - rna , ribosomal rna , ribosome , centrifugation , transfer rna , cytoplasm , neuroblast , embryo , biology , cell , polysome , microbiology and biotechnology , biochemistry , chemistry , gene , neurogenesis
— A critical evaluation of a previously described method of bulk preparation of neuroblasts and spongioblasts from chick embryo cerebral hemispheres furnished the following results.1 A major loss of total RNA occurs during the preparation of the cell fractions. 2 The loss occurred mainly during the dissociation of the tissue and the following centrifugations. 3 The missing RNA's were found in the supernatants after cell centrifugation at low speed. 4 There was a selective loss of transfer RNA amounting to 70–80 per cent relative to ribosomal RNA. 5 The relative proportions of the 29, 18 and 5 s ribosomal RNA's were constant, but there was a decrease in their proportion relative to heavy‐molecular weight, rapidly‐labelled nuclear RNA. 6 The ribosomal RNA's in the low‐speed supernatant could be resedimented at 35,000 g whereas most transfer RNA could not.The results are consistent with a loss of fragments of cytoplasm containing ribosomes and with additional extraction of transfer RNA during the preparation.