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THE COMPOSITION OF MYELIN FROM THE MUTANT MOUSE ‘QUAKING’
Author(s) -
Gregson N. A.,
Oxberry J. M.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01426.x
Subject(s) - myelin , mutant , cerebroside , phospholipid , polyacrylamide gel electrophoresis , biochemistry , biology , chemistry , microbiology and biotechnology , gel electrophoresis , central nervous system , enzyme , endocrinology , gene , membrane
— Myelin was isolated from the brains of adult Quaking mice, a mutant showing a deficiency of myelin in the central nervous system, and normal controls. The mutant myelin was found to have a higher flotation density than that of the control and showed marked differences in lipid composition. The myelin from Quaking mice was found to be deficient in cerebroside and ethanolamine phospholipid. Acrylamide gel electrophoresis of total myelin protein demonstrated a pronounced deficiency of proteolipid protein. The activity of cyclic 2′,3′‐AMP phosphohydrolase was normal.