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DISTRIBUTION OF RIBOSOMAL MATERIAL IN INCUBATED AND ELECTRICALLY STIMULATED CEREBRAL SLICES
Author(s) -
Ornellas M. R.,
Jones D. A.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01393.x
Subject(s) - stimulation , incubation , rna , ribosomal rna , cerebral cortex , in vitro , biophysics , chemistry , biochemistry , amino acid , incubation period , membrane , biology , endocrinology , gene
— The distribution of ribosomal fractions has been examined in fresh cerebral cortex tissue and in slices maintained in vitro both with and without electrical stimulation. The electrical stimulation used was of a type that has previously been shown to diminish amino acid incorporation into protein. Membrane‐bound and free fractions were obtained and the ratio of their RNA contents were, for the control tissue 3, and for the electrically stimulated tissue 1 , 8. Electrical stimulation was found to decrease the Mg 2+ binding affinity of the free. fraction but was without effect on the bound fraction. Stimulation was also found to increase the leakage of soluble protein and RNA from the tissue and its accumulation in the incubation medium.