Premium
RESPIRATORY ACTIVITY OF GUINEA PIG BRAIN NUCLEI
Author(s) -
Mukherjee S. K.,
Narayanaswami A.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01380.x
Subject(s) - citric acid cycle , oxidative phosphorylation , guinea pig , chemistry , oxygen , biochemistry , phosphate , respiration , sucrose , sodium , centrifugation , respiratory system , adenosine triphosphate , glycolysis , citric acid , succinic acid , potassium , antimycin a , metabolism , mitochondrion , biology , endocrinology , botany , organic chemistry , anatomy
— Preparations of guinea pig brain nuclei, obtained by discontinuous gradient centrifugation in sucrose solutions of pH 6.7–6.8, containing 3 mM‐MgCl 2 and phosphate exhibited steady and reproducible oxygen uptake. Oxygen uptake was stimulated 60–70 per cent by glucose, pyruvate, oxalacetate or α‐ketoglutarate and 267 per cent by succinate. This respiratory activity was unaffected by the relative sodium or potassium ion content of the medium and by variations in the concentration of inorganic phosphate. Agents known to inhibit citric acid cycle oxidation, oxidative phosphorylation and glycolysis diminished oxygen uptake, but antibiotics inhibiting nucleic acid or protein synthesis did not. Treatment of the nuclear preparation with DNase decreased respiratory capacity, which was partially restored by the addition of polyacrylic acid.