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EXPERIMENTAL ALCOHOLISM IN RATS: PROTEIN SYNTHESIS IN SUBCELLULAR FRACTIONS FROM CEREBELLUM, CEREBRAL CORTEX AND LIVER AFTER LONG TERM TREATMENT 1
Author(s) -
Jarlstedt J.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01377.x
Subject(s) - cerebellum , cerebral cortex , microsome , leucine , cortex (anatomy) , biochemistry , cerebellar cortex , in vivo , amino acid , medicine , stimulation , mitochondrion , biology , endocrinology , chemistry , in vitro , neuroscience , microbiology and biotechnology
— The incorporation in vivo of [ 3 H]leucine into protein from subcellular fractions was determined in rats chronically ingesting 15 per cent ethanol for 8 months. Mitochondrial, microsomal and cell sap fractions from cerebellum, cortex cerebri and liver were investigated. The results showed a minor over‐all depression of protein synthesis in cerebellum and cortex cerebri and a slight stimulation of the incorporation of leucine into protein from liver subcellular fractions. If the animals were abstinent 24 h before injection of the isotope, the incorporation of labelled amino acids into protein was markedly increased in cerebellum and cerebral cortex but not in liver.