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EFFECTS OF ACETYLCHOLINE ON THE INCORPORATION OF [ 32 P]ORTHOPHOSPHATE IN VITRO INTO THE PHOSPHOLIPIDS OF NERVE‐ENDING PARTICLES FROM GUINEA PIG BRAIN
Author(s) -
Yagihara Y.,
Hawthorne J. N.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01345.x
Subject(s) - phosphatidic acid , acetylcholine , phosphatidylinositol , guinea pig , chemistry , labelling , nucleotide , biochemistry , in vitro , synaptosome , chromatography , phospholipid , biology , endocrinology , membrane , signal transduction , gene
— Guinea pig brain nerve‐ending particles (synaptosomes) were incubated with [ 32 P]orthophosphate in a medium with or without 10 −4 M‐acetylcholine and 10 −4 M‐eserine. Phospholipids were then extracted and separated by chromatography. About 60 per cent of the 32 P was found in phosphatidic acid and about 20 per cent in triphosphoinositide. Acetylcholine significantly increased the specific radioactivity of phosphatidic acid but had no effect on that of phosphatidylinositol or the nucleotide fraction. Labelling of the other phospholipids, including diphosphoinositide and triphosphoinositide, was not altered significantly by acetylcholine. Labelling of the nucleotide fraction and the polyphosphoinositides reached a peak at 40 min, that of phosphatidic acid at 80 min, while that of phosphatidylinositol was still rising at 160 min.