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MYELIN SYNTHESIS IN PERIPHERAL NERVE IN VITRO : SULPHATIDE INCORPORATION REQUIRES A TRANSPORT LIPOPROTEIN 1, 2, 3
Author(s) -
Pleasure D. E.,
Prockop D. J.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01338.x
Subject(s) - myelin , chemistry , microsome , sciatic nerve , in vitro , lipoprotein , biochemistry , endocrinology , medicine , microbiology and biotechnology , biology , cholesterol , central nervous system
— Sciatic nerves from 18‐day‐old chick embryos incorporated 35 SO 4 into myelin sulphatide in vitro . Sulphatide in a microsomal subfraction of the nerve was rapidly labelled with 35 SO 4 , and a lipoprotein fraction in the nerve served to transfer the [ 35 S]sulphatide from the microsomal subfraction to myelin. Puromycin and cycloheximide inhibited the incorporation of [ 35 S]sulphatide into myelin after a lag period of about 2 h. These agents did not alter the rate of appearance of [35S]sulphatide in the microsomal subfraction, and did not diminish the capacity of myelin to take up [ 35 S]sulphatide from the lipoprotein fraction; instead, they appeared to interfere with the incorporation of [35S]sulphatide into myelin by decreasing the available pool of the transport lipoprotein. Partial characterization of the [ 35 S]labelled lipoprotein fraction indicated that it had a density of 1.06–1.08. The lipoprotein was highly aggregated, but, after incubation with SDS and mercaptoethanol, it was dissociated into sulphatide‐containing micelles and proteins.