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THE ACTION OF GAMMA‐HYDROXYBUTYRIC ACID ON CEREBRAL GLUCOSE METABOLISM
Author(s) -
Taberner P. V.,
Rick J. T.,
Kerkut G. A.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01334.x
Subject(s) - in vivo , pentose phosphate pathway , metabolism , carbohydrate metabolism , cerebral cortex , chemistry , respiration , dehydrogenase , glucose 6 phosphate dehydrogenase , endocrinology , medicine , pharmacology , biochemistry , biology , enzyme , glycolysis , anatomy , microbiology and biotechnology
— Experiments have been performed to study the effect of gamma‐hydroxybutyric acid (GHB) on glucose metabolism in vivo and in vitro .1 Administration of GHB (500 mg/kg) is followed by sleep in rats and mice. 2 GHB is shown to increase the 1‐ 14 C/6‐ 14 C ratio in expired CO 2 in mice in vivo by 300 per cent. 3 The same effect is obtained with slices of cerebral cortical grey matter from GHB‐treated rats, where the 1‐ 14 C/6‐ 14 C ratio is increased from 1.72 to 3.63, but not with homogenates of cerebral cortex, nor with slices of kidney or diaphragm. 4 GHB (500 mg/kg, intrapentoneally) specifically increases the activity of glucosed‐6‐phosphate dehydrogenase in vivo in rat and mouse whole brain by 27 per cent. The time course of this effect correlates with the sleeping time in both species. 5 The activity of glucose‐6‐phosphate dehydrogenase is not altered in vitro by high concentrations of GHB. 6 GHB stimulates O 2 uptake by slices of cerebral cortical grey matter by 24 per cent, but it is not itself able to support respiration by the tissue. 7 It is proposed that GHB specifically increases the activity of the pentose phosphate pathway in brain, and that this effect is mediated by an increase in glucose‐6‐phosphate dehydrogenase activity.