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EFFECTS OF ENVIRONMENTAL COMPLEXITY ON AMINO ACID INCORPORATION INTO RAT CORTEX AND HIPPOCAMPUS IN VIVO
Author(s) -
Levitan I. B.,
Mushynski W. E.,
Ramirez G.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01321.x
Subject(s) - hippocampus , cortex (anatomy) , in vivo , hippocampal formation , weaning , cerebral cortex , biology , stimulation , medicine , endocrinology , biochemistry , amino acid , chemistry , neuroscience , microbiology and biotechnology
— Amino acid incorporation in vivo was investigated in the cortex and hippocampus of rats raised in enriched and deprived environments for various periods of time following weaning. At early times after weaning (7 days), the incorporation of l ‐[ 3 H]leucine into all sub‐cellular fractions of both cortex and hippocampus was higher in enriched than in deprived rats. At 16 days, incorporation into synaptosomal sub‐cellular fractions was higher in enriched than in deprived hippocampus, and lower in enriched than in deprived cortex; incorporation into perikaryal fractions of both brain regions was the same in the two groups of animals. Incorporation into subcortical nuclear protein fractions was higher in enriched rats at this time. At 35 days, the only difference between enriched and deprived rats was a lower incorporation into cortical synaptosomal sub‐fractions in the former. Experiments involving double labelling and electrophoresis indicate that there is no stimulation or inhibition of the synthesis of any particular protein in hippocampal nuclear and synaptosomal sub‐fractions of enriched rats. Synaptosomal proteins of cortex have a greater half‐life in enriched than in deprived rats; proteins of perikaryal fractions of cortex, and of all fractions of hippocampus, are turning over at the same rate in enriched and deprived animals.