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THE ACTION OF CALF BRAIN SIALIDASE ON GANGLIOSIDES, SIALOGLYCOPROTEINS AND SIALOGLYCOPEPTIDES
Author(s) -
Heijlman J.,
Roukema P. A.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01315.x
Subject(s) - sialidase , sialic acid , sialoglycoproteins , ganglioside , glycoprotein , biochemistry , chemistry , mucin , endogeny , hydrolysis , oligosaccharide , substrate (aquarium) , chromatography , neuraminidase , enzyme , biology , ecology
— In agreement with other investigators it has been shown that endogenous as well as added gangliosides are a substrate for brain sialidase. The release of sialic acid was enhanced in the presence of Triton X‐100; this might be due to the action of the detergent on the ganglioside micelles. The sialic acid release from endogenous gangliosides was observed over 48 h and compared with the effect of the sialidase on the endogenous glycoproteins. Though the hydrolysis of sialic acid from gangliosides is much faster in the first hours, after 48 h 40 per cent of the total bound sialic was released from both substrates at pH 4.0 and 37°C. Sialoglycopeptides obtained from brain glycoproteins are also metabolized by the sialidase. No effect of Triton X‐100 on this substrate has been observed. From sialoglycopeptides, fractions can be obtained by DEAE‐Sephadex A‐50 column chromatography with a sialic acid content from 8 to 26 per cent. The fractions with a high sialic acid content were about equally active towards brain sialidase as gangliosides. The results agree with the similar turnover rate observed for the carbohydrate chains from gangliosides and glycoproteins, but are in contrast to the observations of other investigators who have stated that glycoproteins are a poor substrate for brain sialidase. In our experiments bovine and ovine submaxillary mucins and sialyl‐lactoses showed only slight activity compared to gangliosides and selected brain sialoglycopeptides.