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THE RELEASE OF AMINO ACIDS FROM THE HEMISECTED SPINAL CORD DURING STIMULATION
Author(s) -
Roberts P. J.,
Mitchell J. F.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01307.x
Subject(s) - stimulation , glutamate receptor , glycine , chemistry , toad , serine , calcium , threonine , efflux , biochemistry , spinal cord , amino acid , endocrinology , medicine , biology , neuroscience , enzyme , receptor , organic chemistry
— Isolated frog or toad hemicords were incubated for 40 min with either [ 14 C]glycine, [ 3 H]GABA, l ‐[ 14 C]glutamate. l ‐[ 14 C]aspartate, l ‐[ 14 C]serine, l [ 14 C]threonine or l ‐[ 3 H]leucine, and the release of these compounds from the cord was measured under resting conditions and during electrical stimulation. Stimulation of spinal roots produced no significant change in the efflux of any of the compounds tested. Direct stimulation of the rostral cord however, produced a large increase in the efflux of [ 14 C]glycine, [ 3 H]GABA, l ‐[ 14 C]glutamate and l ‐[ 14 C]aspartate. These increased effluxes were calcium dependent, the effects of stimulation being reduced in a calcium‐free, or magnesium‐supplemented (10 mM) medium. Stimulation failed to produce an increase in the efflux of l ‐[ 14 C]serine, l ‐[ 14 C]threonine, l ‐[ 14 H]leucine, [ 14 C]mannitol or [ 14 C]urea. These results are consistent with the suggestions that glycine, GABA, glutamate and aspartate may be synaptic transmitters in the spinal cord.