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ZONAL CENTRIFUGATION OF NEURONAL PERIKARYA AND ISOLATION OF NEURONAL MEMBRANES RICH IN ACETYLCHOLINESTERASE
Author(s) -
Kuenzle C. C.,
Pelloni R. R.,
Kistler G. S.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01287.x
Subject(s) - membrane , acetylcholinesterase , centrifugation , endoplasmic reticulum , aché , intracellular , differential centrifugation , chemistry , isolation (microbiology) , biochemistry , cholinesterase , chromatography , biology , enzyme , bioinformatics , pharmacology
— A method is presented that allows the isolation of neuronal perikarya of high purity and in good yield from rat brain. To this procedure is coupled a second isolation step which affords neuronal membranes in preparative quantities. The arrangement of the two isolation steps in tandem ensures that the final preparation has a high degree of purity with respect to both its neuronal origin and its membrane content. The membranes so obtained do not constitute plasma membranes but rather represent intracellular structures that are predominantly derived from endoplasmic reticulum and contain mitochondrial elements as well. During the process of membrane purification AChE is enriched four‐fold while cholinesterase is concurrently eliminated. The present method supplements the procedure reported by M organ , W olfe , M andel and G ombos (1971) for the isolation of synaptosomal plasma membranes. It may also open access to a useful source of brain AChE.