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TIME AND TEMPERATURE DEPENDENT ACTIVATION OF PIG BRAIN GLUTAMINASE
Author(s) -
Svenneby Gerd
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01266.x
Subject(s) - amidohydrolase , glutaminase , arrhenius plot , enzyme , chemistry , phosphate , glutamine , arrhenius equation , boron , biochemistry , enzyme activator , enzyme assay , stereochemistry , biophysics , activation energy , biology , organic chemistry , amino acid
— A time‐dependent activation of the tris‐HC1 enzyme form of pig brain glutaminase (EC 3.5.1.2, L‐glutamine amidohydrolase) by phosphate, phosphate‐borate and carboxylic acids is described. This time‐dependent activation increases with increased protein concentration and also with temperature. The sedimentation behaviour of the various activated enzyme preparations is described. The Arrhenius plot for the triq‐HC1 enzyme is curved, indicating a reversible equilibrium between two enzyme forms, whereas the plot obtained for the phosphate‐borate enzyme was a straight line. The apparent activationenergy has been determined.