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INCORPORATION OF RADIOACTIVE SULPHATE INTO SULPHATIDE DURING MYELINATION IN CULTURES OF RAT CEREBELLUM
Author(s) -
Silberberg D.,
Benjamins Joyce,
Herschkowitz N.,
McKhann G. M.
Publication year - 1972
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1972.tb01248.x
Subject(s) - cerebellum , myelin , in vitro , in vivo , explant culture , medicine , chemistry , endocrinology , microbiology and biotechnology , biology , biochemistry , central nervous system , genetics
— Explants of rat cerebellum obtained 12‐24 h after birth were maintained in culture in Maximow assemblies. About 90 per cent of the cultures myelinated after 10–12 days in vitro . Cultures maintained for varying periods of time were exposed to [ 35 S]Na 2 SO 4 ; labelled sulphatide was recovered from the total homogenate. Preparation of a subcellular fraction with density properties corresponding to those of myelin indicated that labelled sulphatide appeared in this fraction. Cultures which were poorly‐myelinated always exhibited a lower rate of inccrpomtion than well‐myelmated cultures from littermate animals, but the distribution of labelled sulphatide into the ‘myelin’ fraction was similar in the two groups. The rate of incorporation of [ 35 S]Na 2 SO 4 into total sulphatide increased with the duration of the culture, with a low level of incorporation until the seventh day in vitro , followed by a sharp increase in rate up to the 21st day. This pattern resembles that observed for rat cerebellum in vivo .