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A PROCEDURE FOR THE MEASUREMENT OF HEMICHOLINIUM‐3 IN BRAIN TISSUE
Author(s) -
Slater P.,
Stonier P. D.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb12024.x
Subject(s) - physostigmine , medulla oblongata , pons , choline , acetylcholine , tetramethylammonium , chemistry , atropine , central nervous system , tetraethylammonium , medicine , endocrinology , biology , biochemistry , potassium , ion , organic chemistry
— The natural fluorescence of hemicholinium‐3 (HC‐3) was used to provide a method for determining its concentration in brain tissue following intraventricular administration of the compound to rats and rabbits. When hemicholinium‐3 was perfused through the cerebral ventricles of the rabbit, the highest concentration was found in the pons‐medulla oblongata. The effect of a number of compounds on the uptake of hemicholinium‐3 in the brain was studied in rats. Choline and tetramethylammonium significantly reduced the uptake of HC‐3 from the cerebral ventricles but tetraethylammonium, physostigmine and atropine had no effect. The results are discussed in relation to the known effects of hemicholinium‐3 in the central nervous system.