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FRACTIONATION OF GOLDFISH BRAIN AMINOACYL‐TRANSFER RNA AT THE MICROGRAM LEVEL 1
Author(s) -
Kaplan B. B.,
León V. De,
Sirlin J. L.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb12014.x
Subject(s) - transfer rna , chromatography , fractionation , microgram , chemistry , resolution (logic) , rna , biochemistry , in vitro , artificial intelligence , computer science , gene
— The methylated albumin‐kieselguhr column first employed by SUEOKA and YAMANE (1962) to fractionate tRNA was modified and scaled down by a factor of 100 to permit the separation of 1 y% of goldfish brain tRNA. Double labelling, co‐chromatography of single aminoacylated tRNA species demonstrated that the column can characterize isoaccepting tRNAs without serious loss of resolution. The procedure is simple and reproducible and can be useful for the rapid scanning of tRNAs from limited amounts of biological material. Further scaling down of the procedure appears to be feasible.