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STUDIES ON SOLUBLE PROTEINS RELEASED FROM THE SYNAPTIC VESICLES OF RAT BRAIN CORTEX
Author(s) -
Matsuda T.,
Saito K.,
Katsuki S.,
Hata F.,
Yoshida H.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb12001.x
Subject(s) - synaptic vesicle , vesicle , acetylcholine , chemistry , cerebral cortex , synaptosome , ultracentrifuge , synaptic membrane , biophysics , biochemistry , membrane , biology , neuroscience , endocrinology
— The soluble proteins released from the synaptic vesicles of rat cerebral cortex were studied. One fraction (D 4 ) of these proteins was released in parallel with release of acetylcholine when synaptic vesicles were incubated at 37°C for 10 min in isotonic medium. Another fraction (Dj) was liberated from synaptic vesicles when their membranes were ruptured by mild treatment under hyposmotic conditions and freeze‐thawing after release of D 1 fraction. Fractions D 1 and D 2 contained 12 and 9 per cent, respectively, of the total protein in the synaptic vesicles. Some properties of these fractions were investigated by zone electrophoresis and ultracentrifugation, and by measuring their binding capacities for [ 14 C]acetylcholine and various enzyme activities related to acetylcholine metabolism.