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DISC ELECTROPHORETIC SEPARATION OF PROTEINS IN NEURONAL, GLIAL AND SUBCELLULAR FRACTIONS FROM CEREBRAL CORTEX
Author(s) -
Packman P. M.,
Blomstrand C.,
Hamberger A.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb11975.x
Subject(s) - cerebral cortex , isozyme , electrophoresis , biochemistry , labelling , cortex (anatomy) , polyacrylamide gel electrophoresis , polyacrylamide , biology , lactic dehydrogenase , chemistry , microbiology and biotechnology , enzyme , neuroscience
— Soluble proteins were studied in preparations from rabbit brain cortex enriched in neuronal or glial cells and in subcellular cortical fractions. Analytical polyacrylamide gels were used for acidic (pH 9‐5) and basic (pH 4‐3) proteins and qualitative and quantitative differences are described. The isozymes of lactic dehydrogenase, brain specific proteins and radioactive labelling patterns were used to characterize some soluble proteins.