Premium
THE INFLUENCE OF INTRAVENTRICULARLY INJECTED AMINO ACID EXCITANTS ON THE LABELLING OF ENDOGENOUS BRAIN AMINO ACIDS FROM [U‐ 14 C]ACETATE IN NEMBUTALIZED MICE
Author(s) -
Watkins J. C.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb03748.x
Subject(s) - glutamine , amino acid , chemistry , alanine , metabolism , glutamate receptor , biochemistry , labelling , citric acid cycle , endogeny , glutamic acid , tricarboxylic acid , stereochemistry , receptor
Mice were anaesthetized with nembutal and the effects of intraventricularly injected excitant amino acids on [U‐ 14 C]acetate metabolism were investigated. The natural excitant amino acids, l ‐glutamate and l ‐aspartate, reduced the incorporation of 14 C from [U‐ 14 C]acetate into glutamine, GAB A and possibly alanine. The synthetic excitant amino acid, N ‐methyl‐ d ‐aspartate caused a reduction in the incorporation of 14 C from intraventricularly injected [U‐ 14 C]acetate into all of the brain amino acids labelled by [U‐ 14 C]acetate within 5 min. It is suggested that these effects may be due to changes in pool sizes of tricarboxylic cycle intermediates, to inhibition of acetyl‐CoA formation, or both. Differences in the metabolic effects of the synthetic and natural excitants are interpreted in terms of the uptake of the natural amino acids into glutamine‐forming pool(s) of glutamate metabolism.