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A MICRO‐SCALE PROCEDURE FOR THE PREPARATION OF SUBCELLULAR FRACTIONS FROM INDIVIDUAL AUTONOMIC GANGLIA
Author(s) -
Giacobini E,
Hökfelt T,
KerpelFronius S,
Koslow S. H,
Mitchard M,
Noré B
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb00560.x
Subject(s) - denervation , membrane , free nerve ending , electron microscope , chemistry , biophysics , vesicle , autonomic ganglion , aché , mitochondrion , enzyme , biochemistry , anatomy , biology , acetylcholinesterase , neuroscience , physics , optics
— A microscale modification for the preparation of subcellular fractions employing milligram and submilligram amounts of neuronal tissue (brain nuclei and autonomic ganglia) is described. Electron microscope characterization and enzymic studies were carried out on the six subcellular fractions of sympathetic ganglia of cat thus prepared. The synaptosomal preparations obtained from individual ganglia were poorer in their nerve ending content than those obtained from brain by previous investigators. The highest RSA for AChE was found in layer L 2 which was rich in membranes and vesicle components. ChAc activity was also highly concentrated in layers L 2 and L 3 (membranes, nerve ending‐like particles, mitochondria and ‘ghosts’). MAO activity was particularly high in the layers L 4 and L 5 which contained a large number of mitochondria. Layer L 1 (membrane fragments) and particularly layer L 6 which contained mainly collagen fibres, were low in activity of all three enzymes. After preganglionic denervation, both ChAc and AChE activities were significantly reduced in the purest nerve ending fraction, L 3 while MAO activity was practically unchanged.