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ON ARABINOSE AS A CONSTITUENT OF HYALURONIC ACID FROM BOVINE BRAIN
Author(s) -
Katzman R. L.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb00217.x
Subject(s) - arabinose , hyaluronic acid , chemistry , polysaccharide , chromatography , xylose , bromide , hydrolysate , biochemistry , organic chemistry , biology , fermentation , hydrolysis , anatomy
(1) W ardi , A llen , T urner and S tary (1966) and M argolis (1967) have reported that arabinose is a component of hyaluronic acid from mammalian brain. (2) In the present study, total acidic polysaccharide and hyaluronic acid fractions were isolated from lipid‐extracted and proteolysed bovine brain by precipitation with cetyltri‐methylammonium bromide. These fractions were analysed for arabinose by paper chromatography of deionized hydrolysates and by gas‐liquid chromatography of per(trimethylsilyl)ated methanolysates. (3) Two pentoses, xylose and ribose, were detected. Arabinose was analytically undetectable in both polysaccharide fractions, but was easily detected in a control polysaccharide containing 0‐1% (w/w) arabinose. Arabinose, if present in hyaluronic acid from bovine brain, constitutes less than 0.1 mol per mol of hyaluronic acid (molecular weight 1.5 x 10 6 daltons).