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GLYCOL SULPHATE ESTER FORMATION FROM [ 14 C]NORADRENALINE IN BRAIN AND THE INFLUENCE OF A COMT INHIBITOR
Author(s) -
Sugden R. F.,
Eccleston D.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb00203.x
Subject(s) - pyrogallol , chemistry , amine gas treating , catechol , derivative (finance) , catechol o methyl transferase , norepinephrine , transferase , metabolism , metabolite , chromatography , biochemistry , medicinal chemistry , stereochemistry , dopamine , organic chemistry , endocrinology , enzyme , biology , allele , financial economics , economics , gene
— A method is described allowing the identification and separation of the sulphate esters of the glycol metabolites of [ 14 C]noradrenaline injected into the lateral ventricle of the rat. The esters of both the 3,4‐dihydroxy derivative and the 3‐methoxy‐4‐hydroxy derivative are formed in substantial amounts. It is suggested that the quantity of the former may have been underestimated in the past. Contemporaneous administration of pyrogallol, a catechol‐O‐methyl transferase inhibitor, with [ 14 C] noradrenaline leads to a considerable fall in 3‐methoxy‐4‐hydroxyphenylglycol sulphate without a rise in 3‐4‐dihydroxyphenylglycol sulphate although free 3‐4‐dihydroxyphenylglycol rises significantly. It is proposed that the latter may be an index of intraneuronal metabolism of noradrenaline and 3‐methoxy‐4‐hydroxyphenylglycol that of released amine.