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ANTIGEN—ANTIBODY CROSSED ELECTROPHORESIS OF WATER‐SOLUBLE RAT BRAIN ANTIGENS
Author(s) -
Bock Elisabeth,
Mellerup E. T.,
Rafaelsen O. J.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb00199.x
Subject(s) - antigen , antibody , chemistry , electrophoresis , immunology , microbiology and biotechnology , biology , chromatography
— A method of separation by antigen‐antibody crossed electrophoresis was applied to the study of water‐soluble rat brain antigens. Five different rat brain preparations were used as antigens for immunization. The extract from Triton X‐100 treated brains gave the best antibody response. An antiserum containing 27 precipitating antibodies was obtained. The preparation of antigens for immunoelectrophoresis was investigated. Treatment with demineralized water gave the highest number of antigen‐antibody precipitates. Treatment with detergents and sonication gave a higher total protein yield, but the number of precipitates was unaffected.