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THE SUBCELLULAR LOCALIZATION OF HISTAMINE AND HISTAMINE METHYLTRANSFERASE IN RAT BRAIN 1
Author(s) -
Kuhar M. J.,
Taylor K. M.,
Snyder S. H.
Publication year - 1971
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1971.tb00014.x
Subject(s) - histamine , histamine n methyltransferase , homogenization (climate) , medicine , chemistry , endocrinology , sucrose , serotonin , biochemistry , biology , histamine h2 receptor , receptor , antagonist , biodiversity , ecology
Abstract— We have examined the subcellular localization of histamine and histamine methyl‐transferase ( S ‐adenosylmethionine: histamine 7V‐methyltransferase; EC 2.1.1.8) in rat brain. The highest levels of histamine and histamine methyltransferase activity were found in the hypothalamus. A large proportion of hypothalamic histamine and histamine methyltransferase activity was found in particles with sedimentation properties in sucrose gradients similar to synaptosomes storing norepinephrine and serotonin. Histamine displayed a bimodal distribution in sucrose gradients. A substantial amount of a tracer dose of [ 3 H]histamine added to hypothalamic homogenates at 4°C was bound to particulate fractions, suggesting that endogenous histamine may redistribute and bind to subcellular fractions during homogenization. The second, lighter peak of histamine in sucrose gradients was thought to be due to histamine that redistributed during homogenization.